Klebsiella pneumoniae is an important pathogen in nosocomial and community-acquired UTIs. It is showing a
high resistance towards many drugs. This study aimed to estimate the percentage of K. pneumonia in Hospital acquired and
Community acquired UTI, drug resistance, ESBL, and detect the resistance genes (bla KPC, bla CTXM, bla SHV) and presence
of mutation in (bla KPC). A total of (200) urine samples were collected; K. pneumonia was cultured and isolated on MacConkey
agar, antibiotic sensitivity testing and ESBL detection were performed by Vitek 2 system, then DNA was extracted, PCR and
sequencing for KPC gene were done. There is 7% (14 isolates) of K. pneumonia (50% HAUTI and 50% CAUTI) within
21.43% positive ESBL were obtained. Percentage of positive bla SHV and bla CTXM genes were 100%, while positive bla KPC
gene was 42.8%. KPC gene sequencing show high rate of mutation in comparison between positive isolates reach to 50
different nucleotides. This work has significantly increased the knowledge on the Klebsiella pneumonia causing HAUTI and
CAUTI patients.
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4-2022
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