Background: Bladder carcinoma is the 9th most common
malignancy. Papillary urothelial carcinoma represents about
45% of all primary bladder tumors.
Objectives: To compare between the immunohistochemical
staining pattern of (CK20 and p16) of (non-neoplastic urinary
bladder urothelium, low and high grade papillary urothelial
carcinoma) and its relation to tumor grade and invasiveness.
Methods: It was a retrospective study. Formalin fixed paraffin
tissue blocks of papillary urothelial carcinoma for 48 patients,
and an equal number of non-neoplastic cases were collected.
Immunohistochemical marker study of two markers (P16 and
CK20) staining pattern was performed.
Results: CK20 stain was positive in (68.8%) of cases, while
only (10.4%) of the controls was positive, with a p value of
(0.002). p16 stain was positive in (70.8%) of cases, whereas its
positivity in the controls was only (20.8%), and the p value was
(0.003). CK20 was positive in 5 invasive cases but negative in
12 invasive cases. On the other hand CK20 was positive in 28
noninvasive cases and negative in only 3 noninvasive cases
with a p value of (0.000). Significant difference (in favor for the
non-invasive cases) in p16 expression was found between
invasive and non-invasive urothelial carcinoma (p value 0.018).
Conclusion: Both markers (CK20 and P16) are adequate and
useful in assessing bladder biopsies and both can be used in
routine practice to confirm the diagnosis of both low and high
grade papillary urothelial carcinoma and P16 positivity may
indicate a good prognosis in papillary urothelial carcinoma
Background: Bladder carcinoma is the 9th most common
malignancy. Papillary urothelial carcinoma represents about
45% of all primary bladder tumors.
Objectives: To compare between the immunohistochemical
staining pattern of (CK20 and p16) of (non-neoplastic urinary
bladder urothelium, low and high grade papillary urothelial
carcinoma) and its relation to tumor grade and invasiveness.
Methods: It was a retrospective study. Formalin fixed paraffin
tissue blocks of papillary urothelial carcinoma for 48 patients,
and an equal number of non-neoplastic cases were collected.
Immunohistochemical marker study of two markers (P16 and
CK20) staining pattern was performed.
Results: CK20 stain was positive in (68.8%) of cases, while
only (10.4%) of the controls was positive, with a p value of
(0.002). p16 stain was positive in (70.8%) of cases, whereas its
positivity in the controls was only (20.8%), and the p value was
(0.003). CK20 was positive in 5 invasive cases but negative in
12 invasive cases. On the other hand CK20 was positive in 28
noninvasive cases and negative in only 3 noninvasive cases
with a p value of (0.000). Significant difference (in favor for the
non-invasive cases) in p16 expression was found between
invasive and non-invasive urothelial carcinoma (p value 0.018).
Conclusion: Both markers (CK20 and P16) are adequate and
useful in assessing bladder biopsies and both can be used in
routine practice to confirm the diagnosis of both low and high
grade papillary urothelial carcinoma and P16 positivity may
indicate a good prognosis in papillary urothelial carcinoma
paient.
(FULL ARTICLE LINK) Read more ...
2017/9
|
|
|