The Impacts of Interferon Gamma Gene Polymorphism on BNT162b2 Induced Antibody Response
حيدر صباح كاظم
Authors : Israa Ali Fadhil Alameri, Haider Sabah Kadhim
Background: Severe acute respiratory syndrome the coronavirus-2 that is responsible for the complicated, highly variable severe acute respiratory syndrome known as coronavirus disease 2019 was found in China in December 2019. Pfizer and BioNTech have teamed up to developed a lipid nanoparticle containing a nucleoside-modified messenger RNA encodes the receptor-binding domain of the spike protein of severe acute respiratory syndrome the coronavirus-2, which is a crucial target of neutralizing antibodies. Objectives: This study was aimed to investigate the role of interferon gamma T/A gene polymorphism +874 (rs2430561) in immune response to coronavirus disease 2019BNT162b2 through:Measuring anti spike-1 IgG serum level by Enzyme-linked immunosorbent assay.Studying the single nucleotide polymorphism in interferon gamma T/A +874 gene, using amplification refractory mutation System-polymerase chain reaction. Subjects and Methods: One hundred and eighty BNT162b2 vaccinated healthy participants aged above eighteen years were included in a cross-sectional study 21–30 days following the booster dose. Deoxyribonucleic acid was extracted from blood samples then genotypes were detected by amplification refractory mutation System (ARMS-PCR). Determination of IgG levels by Enzyme-linked Immunosorbent Assay against spike protein-1of receptor binding domain protein. Results: This study showed a strong significant difference in distribution of interferon gamma genotypes and immune responseto BNT162b2 vaccine (P value 0.032), the frequency of homozygous wild genotypeTT was higher in those with good antibody response unlike homozygous mutant AA genotype was higher in weak antibody response category.Furthermore, there were highly significant association between sex and genotypes frequency, homozygous mutant genotype (AA) was higher in males 43.70% than in females 16.90% (P. value less than 0.001). Conclusions: Interferon gamma +874 T/A may be associated with BNT162b2 vaccine unresponsiveness. That is, substantial proportion of subjects who carry A allele showed poor antibody response after vaccination, while T allele showed potent association with high levels of anti-S1 IgG titer.

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