Objective: This study aimed to investigate the possible anti angiogenic activity of Vitex agnus castus leaves extracts
Methods: The leaves were dried and grounded into fine powder, and extracted sequentially with Chloroform, methanol and water. Ex-vivo rat aorta
anti angiogenic assay was used to identify the most anti angiogenic extract, active extract has been chosen for dose response testing, by preparing
serial concentrations of that extract and applied on aorta rings of the rat, the active extract was tested on human umbilical ventricular endothelial
cell line as in vitro assay. Free radical scavenging activity has been tested by 1,1-Diphenyl-2-picrylhydrazyl (DPPH) to discover which extract has the
highest free radical scavenging activity and as a mean to elucidate the possible mechanism of extract action.
Results: Methanol extract has the highest percentage of blood vessels growth inhibition in comparison to other extracts P˂ 0.05. However, there
were no significant differences between chloroform and water extract P˃ 0.05. Methanol extract showed dose dependant inhibitory activity on rat
aorta assay the IC50 was (21.73 µg/ml). Methanol extract showed dose response activity against human umbilical ventricular endothelial cells
(HUVEC) and IC50 was (42.09µg/ml). Methanol extract of Vitex agnus castus leaves showed the most potent free radical scavenging activity in
comparison to other extracts; IC50 was (126.79µg/ml).
Conclusion: The results showed that methanol extract of Vitex agnus castus has potential anti angiogenic activity and this activity may be due to the
high activity in free radical scavenging capability.
(FULL ARTICLE LINK) Read more ...
Int J Pharm Pharmace Sci 2014
|
|
|
