From October 2010 to February 2011, 124 high vaginal swabs were collected, 88 swabs from different
women with contracep ves and 36 swabs from women with-out all of which were suffering from
vulvovaginitis. The specimens were collected from two hospitals in Iraq; Al-Kadhymia Teaching Hospital in
Baghdad and Al-Batool Teaching Hospital in Diyala. Fifty-five specimens from 124 with high vaginal swabs
were identified as Candida spp. the percentage was 44.35%. Forty out of 88 specimens from
contraceptive users showed positive results for Candida spp., whereas 15 out of 36 specimens from noncontraceptive
users showed positive results for Candida spp. of which 35(63.6%). The isolates were
identified as Candida albicans, whereas (36.4%) were iden fied as non-albicans spp., of which 17 (30.9%)
were C. glabrata, while 3(5.5%) were C. tropicalis the statistical analysis showed that there were high
significant differences (P<0.01) between Candida spp. concerning contraceptives. The isolation rate of
Candida spp. were higher (P<0.01) among oral contracep ve pills (OCP) which was 47.05% than
intrauterine contraceptive device (IUD) which was 43.47%. Candida albicans was higher among IUD users
(80%) than OCP (50%). Among non- C. albicans spp., C. glabrata was the most (frequently in all
contraceptive users from non-albicans spp.), and it's equal to C. albicans (50%) among OCP users. C.
tropicalis was isolated from non contracep ve users (20%). Transmission electron microscope
examination showed that C. albicans, C. glabrata and C. tropicalis have the ability to adhere to vaginal
epithelial cells and C. albicans was the most adherent more than the other two species. By using dilution
method the minimum inhibitory concentration (MIC) of (ketoconazole, clotrimazole, metronidazole and
nystatin) for both C. albicans and C. tropicalis was 12.5 μg/ml and 6. 25 μg/ml in C. glabrata except
clotrimazole which gave MIC 12.5 μg/ml. Minimum fungicidal concentration (MFC) was 50 μg/ml for all
antifungal.
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2013
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