Neonatal Sepsis is a bacterial infection of the blood in a neonate and an infant younger than 4 weeks of age. Molecular methods may display usefulness due to the rapidity and the small sample volume required for analysis. These techniques, exhibitionist the presence of microbial DNA in the sample, which based on amplification or hybridization. The goal of this investigation to identify the pattern of organisms in neonatal sepsis using Polymerase Chain Reaction-Based 16S rRNA Sequencing in Al-Imammain Al-Kadhmain Teaching Hospital. Blood samples were inoculated into a blood culture bottle and incubated at 37°C under aerobic conditions, DNA extracted from blood samples then 16S rRNA PCR amplification and Sanger sequencing (PCR/Sanger-Seq) was used for detection of bacterial pathogens. Positive blood cultures were 41(82%) infants with sepsis, according to 16Sr RNA gene conventional PCR assay results improved that 40 (80%) out of 50 samples were PCR positive. These positive samples were analyzed using 16S rRNA gene sequencing, discordant identification results between API system and sequencing were found in 15 samples, and one sample was identified Genus/species achieved only by sequencing as a Pantoea agglomerans, sequencing was the only method that identified two bacterial strain belong Achromobacter xylosoxidans and three strains belong to Enterobacter cloacae. In conclusion, the use of PCR sequence analysis in diagnosis neonatal sepsis can be highlight rare types of bacteria that lead to this disease, which helps to focus on these species and to provide appropriate treatment.
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5/9/2018
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