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Detection of Escherichia coli O157:H7 Strain by conventional and molecular methods from diarrheal children in Baghdad.
ثناء رشيد عبد الرحمن
Authors : Thanaa R. Abdulrahman PhD
Escherichia coli O157:H7 is a pathotype of Enterohemorrhagic Escherichia coli (EHEC) which is considered as a public health problem bacteria induced food-borne diarrheas, bloody diarrhea and hemolytic uremic syndrome that occurring at any age group. Three hundred stool samples obtained from children with bloody diarrhea, their age ranged from one month to five years whom visited or admitted as “out-patients” to Al-Eskan pediatrics hospital, Al-Kadhumia pediatric hospital or from private clinic in Baghdad. EHEC was found in 37 (12.33%) patients. The highest rate 18 (48.64%) were in infants aged (3-12) months and 12 out of 37(32.43%) in age group (13-24) months. A 25 isolates were positive on Sorbitol MacConkey Agar with Cefixime and Tellurite (SMAC-CT). Commercial latex agglutination test revealed that E.coli:O157:H7 was found in 14(56%) out of 25 culture positive cases while the other 11 (44%) isolates were E.coli O157: H˗. The results of PCR amplification of (vt1 gene) showed that 37 (12.33%) out of 300 stool samples were produce (130 pb) amplified band. All culture positive sample (n=25) were also positive by PCR.

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2016