ABSTRACT Background: Acute lymphoblastic leukemia (ALL) is a malignant expansion of immature lymphoid cells that results from multi-step genetic changes in a single lymphoid progenitor cell. Flowcytometric Immunophenotyping of childhood acute lymphoblastic leukemia (ALL) plays an important role not only in the diagnosis and classification of B and T cell lineages, but also in predicting the outcome. B-lymphoid blasts from acute lymphoblastic leukemia (ALL) have been shown to express functional CD123 and the CD40 ligand stimulates proliferation of precursor-B (pre-B) ALL by upregulating CD123 expression. Aims of the study: 1. Detect expression of interleukin 3 receptor (alpha chain) CD123 in childhood acute lymphoblastic leukemia cases (ALL). 2. Correlate the expression of CD123 with hematological parameters and to the induction chemotherapy. Patients and Methods: Thirty three patients under 15 years of age admitted to the Children’s Welfare Teaching Hospital (CWTH) for diagnosis and treatment. Haematological parameters including Hb, WBC count, and platelet count were obtained from the file records of the patients. Flowcytometry Immunophenotyping was done for interleukin 3 receptor alpha subunit (CD123),CD19 and CD3 were investigated in those ALL patients by using four –color Cyflow® Cube 6 flow cytometry device (PartecCyflow®, German). Results: In B-ALL CD123 expression was positive in 21 out of 23 patients (91.3%), while in T-ALL no patient express CD123. There was 95.6% of B-ALLs had achieved complete remission after first course of induction therapy, versus 80% of T-ALL. In B-ALLs all 21 patients who were CD123 positive had responded to induction remission, moreover the only one case who did not respond to therapy was CD123 negative and PAS negative; p-value < 0.05. Conclusion: CD123 is frequently expressed in pediatric ALLs blasts. CD123 was exclusively expressed in B-lineage with absent expression in T-lineage ALLs. CD123 is significantly associated with good prognostic indicators and good treatment response to initial induction therapy.
KEYWORDS: Acute lymphoblastic leukemia (ALL), CD3, CD19, CD123, Flowcytometry, Immunophenotyping, Interleukin 3 receptor.
(FULL ARTICLE LINK) Read more ...
2016
|
|
|
