To develop a new rapid and sensitive HPLC method for measurement of vitamin A and β-carotene in sera
of healthy individuals. This study was conducted at chemistry and biochemistry department, college of
medicine, Al-Nahrain University, Baghdad, Iraq from July2014 to March 2015; a cross-linked polymer was
synthesized by condensation reaction between mixture of glycerol and triethanol amine with maleic
anhydride. The resulted polymer have high rigidity and easily grinded, with high stability and used as
stationary phase for HPLC column. Vitamin A and β-carotene were measured using by improved
gradient reversed phase HPLC technique. Reproducible determination with highly sensitive detection was
attained by HPLC with UV-Visible detection. The detection limit of vitamin A and β-carotene were 0.05and
0.1μg/ml respectively. Fifty healthy volunteers aged 25.45±6.54 years were included in this study. All
participant and exclude taking supplement of multi vitamins for the last two months. The method gives a
good linearity ranges between 0.05 to 15μg/ml for Vitamin A and β-Carotene. The CVs% of within-day
precision for vitamin A was 1.41-2.42% and β-carotene was 0.81-1.17%. While the analytical recoveries
were98.2% for vitamin A and 96.8% for β-carotene.
KEYWORDS: triethanolamine-glycerol-maleate, vitamin A, β-carotene, HPLC.
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Int J Pharm Bio Sci 2016 April.; 7(2): (B) 350 - 358
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