Toxoplasmosis is the general term for infection and disease in man and animal
caused by a parasite called Toxoplasma gondii. There are six members in the IL-17
cytokine family, including IL-17A (commonly referred to as IL-17).In
Toxoplasmosis the role of IL-17 was involved in the development and early
recruitment of neutrophils, which are essential to clear the parasites during initial
stages of infection. This study was carried out to diagnosis the Toxoplasma
lymphadenitis and to determine the role of IL-17 in this disease. A total of 45
lymph node tissue samples (paraffin block) were enrolled in this study. Three
sections were made from the paraffin-embedded tissue blocks, one section for H&E
staining. Two sections were processed by immunohistochemistry (IHC) procedure.
The first for diagnosis Toxoplasma antigen (P30) while the second section for
diagnosis IL-17byusing specific immunohistochemistry (IHC) detection kits. From
45 patients who examined by H&E, only 25 patients had evidence of Toxoplasma.
The lymph nodes with histological evidence of Toxoplasmosis showed positive
Immunohistochemical Toxoplasma antigen expression, but, neither reactive lymph
nodes nor normal ones showed positive immunohistochemical Toxoplasma antigen
expression. Immunohistochemical expression of IL-17 was assessed by two
methods. The first one is represented by the percentage of inflammatory cells that
express the marker IL-17, and the second method is named "IL-17 score" In this
study we concluded there are highly significant expressions in levels of IL-17 by
immunohistochemistry assay in both the percent and score methodswhich can give
an idea about the association between IL-17 and toxoplasma lymphadenitis.
(FULL ARTICLE LINK) Read more ...
(Volume 4 Number 12 (2015
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