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ANALYSIS OF SOME BIOMEDICAL COMPOUNDS USING TRIETHANOLAMINE-GLYCEROL-MALEATE COPOLYME AS HPLC STATIONARY PHASESR
Authors :
ABSTRACT A cross-linked polymer was synthesized by condensation reaction between mixture of glycerol and triethanol amine with maleic anhydride. The resulted polymer have high rigidity and easily grinded, with high stability and used as stationary phase for HPLC column. Vitamin E was analyzed with triethanolamine-glycerol-malate column with isocratic eluention 100% acetonitrile as a mobile phase with flow rate of 1ml/min and UV detection of 229nm, some amino acids (tyrosine, tryptophane and phenylalanine) were also examined with this column with isocratic eluention 15% phosphate buffer adjusted pH at 6 in 85% ethanol as a mobile phase with flow rate of 1ml/min and UV detection of 245nm. Furthermore some of drugs, such as amiloride, furosemide, and atenolol were analyzed with this stationary phase. The eluent 20% phosphate buffer at pH 8 in 80% methanol with UV detection at 233 nm has given a good analysis for the drugs using isocratic elution. The retention times for furosemide, atenolol and amiloride were 4.6 min, 7.1 min and 9.2 min, respectively. The separation was improved by with elution gradient (0-20) % phosphate buffer in methanol. Good recoveries were obtained for the drugs ranged from 95-98% by using triethanolamine-glycerol-maleate column. The results obtained were compared with commercial column ODS-C18. The method of analysis was applied for determination of the drugs in pharmaceutical tablets as well as in serum samples.

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2015