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An increased degree of oxidative DNA damage in Iraqi males with breast tumors
Authors :
Reactive oxygen species (ROS) play a pathogenic role in carcinogenesis by inducing oxidative DNA damage, ROS-induced DNA oxidation leads to a multitude of modifications to DNA bases, with 8-hydroxy-2-deoxyguanosine (8-OHdG) representing the most frequent one. The current study aims to assess the oxidative DNA damage in Iraqi males with breast tumors using 8-hydroxyl-2-deoxygunosine (8-OHdG) levels and numbers of DNA lesions. Serum, urine samples and post operative tumor specimens were taken from 20 males with newly diagnosed breast tumors (age 24-65 years) and serum and urine samples of 20 healthy controls (age range 24-65 years). The level of 8- OHdG was measured by ELIZA and numbers of DNA lesions was estimated by special formula. Our data showed a statistically significant increased (p< 0.0001) in the mean levels of serum and urine 8-OHdG and DNA lesion in benign and malignant breast tumors as compared to the control groups with augmented elevations in these markers in malignant breast tumor tissues as compared to the benign ones. Conclusion: - Oxidative DNA damage is significantly associated with breast cancer risk, both serum and urine 8-OHdG levels are useful markers for DNA modification in the cells. 8-OHdG levels contribute to the detection of the degree of DNA damage in persons with breast tumors.

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September 2015