The study aimed to investigate the possible antiangiogenic activity of Commiphora molmol oleo-gum-resin extracts. The oleo-gumresin
was grounded to fine powder, and extracted using sequential extraction, using the following solvents in increasing order of
polarity: chloroform, methanol and de-ionized distilled water. Ex-vivo rat aorta antiangiogenic assay was used to identify the most
antiangiogenic extract, active extract has been chosen for dose response study, and the active extract was tested on human
umbilical ventricular endothelial cell line as in vitro study. Free radical scavenging activity has been tested by 1,1-Diphenyl-2-
picrylhydrazyl (DPPH) to detect which extract has the highest free radical scavenging activity to help determine the possible
mechanism of extract action. Methanol extract was the most biological active extract in terms of percentage of blood vessels growth
inhibition in comparison to chloroform and water extracts (P˂ 0.05). Also, there were significant differences between methanol,
chloroform and water extract (P< 0.05). Methanol extract concentrations showed significant dose dependent inhibition activity
(P<0.05) on rat aorta assay and IC50 was (20.78μg/ml). IC50 value was calculated from the linear regression equation. Methanol
extract indicates significant dose related inhibitions against human umbilical ventricular endothelial cells (HUVEC) and IC50 was
(77.59μg/ml). Methanol extract had the highest free radical scavenging activity in comparison to chloroform and water extract. The
IC50 of DPPH for methanol extract was (109.76 μg/ml). Commiphora molmol extracts showed potential antiangiogenic activity and
this activity may be due to the high activity in free radical scavenging capability. Highest effect was observed by the methanolic
extract.
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30-06-2015.
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